They had free access to regular mouse chow and water during the acclimation period. The modified hole board mhb is used to assess multiple dimensions of unconditioned behavior, mainly in mice and rats 1. Female c57bl6 or sjl mice 8 to 12 weeks of age are acclimated to our facility for at least 7 days. The exploratory behaviour of rats in the hole board apparatus. Below are comments submitted by users for the quiz of mice and men test. Both frequency and duration of spontaneous elicited hole poking behavior are then measured during a short period of time. Test questions include text excerpts with questions, vocabulary in context questions and character trait matching questions. An oral glucose tolerance test ogtt, in which the mice are challenged with a bolus of glucose and blood glucose and insulin levels are measured across a two hour time course is performed one week prior to the initiation of the hfd challenge. The exploratory behaviour of rats in the holeboard apparatus. To establish the mhb test, we investigated the behavior of wistar rats that had been bred selectively for innate emotionality on the elevated plus maze epm.
Mice were group housed upon arrival four per cage and allowed to acclimate to our facility for an average of 2 weeks prior to behavioral testing. Of mice and men chapter 1 quiz pdf of mice and men chapter 1 quiz test your knowledge. Qualey part 1 multiple choice 15 points circle the letter of the answer that best answers each question. This protocol describes the modified hole board mhb, which combines features from a traditional hole board and open field and is designed to measure multiple dimensions of unconditioned behavior in small laboratory mammals e. Results of the open field test under different light.
Changes in headdipping behavior in the holeboard test. Extract of bryophyllum pinnatum in mice 1salahdeen h. In c57bl6 mice, eae is induced according to the protocol eae induction by active immunization in c57bl6 mice in sjl mice, eae is induced according to the protocol eae induction by active immunization in sjl mice mice are scored daily for eae severity on the scale of 05. Mouse genome informatics the jackson laboratory bar harbor, maine december 2005 table of contents subject index users guide. July 14, 2005 when memory is lost due to dementia such as alzheimers disease, is it gone for good scientists probed that idea in lab tests on mice. Introduction drug development requires testing of novel compounds in animal. The modified hole board measuring behavior, cognition and. Of mice and men multiple choice test pdf 50 question multiple choice test for of mice and men by john steinbeck. Neuropharmacological effects of aqueous leaf extract of. The subject mouse can receive visual, auditory, and olfactory stimuli from the cage mate, but physical interaction is blocked. Many unconditioned tests, such as the open field, potentially confound general locomotor activity with exploration. Its now time for my students to demonstrate what theyve learned by completing the summative assessment, unit test of mice and men by john steinbeck.
Review of 100 recent articles from the past few years in relevant journals using open field or plus maze tests. What type of animal does lennie want to take care of when george and him. Holeboard discrimination learning in mice wiley online library. Of mice and men unit test test key language arts 10 mr. Abstract effects of aqueous leaf extracts of bryophyllum pinnatum aebp on some neuropharmacological activities were studied in mice. Moreover, we also found that treatment with anxiolytics or anxiogenics and exposure to acute restraint stress affected headdipping behavior. The study was conducted using a wooden board measuring 20 cm by 40 cm with sixteen evenly spaced holes. As to holeboard assay, by means of mva, we have demonstrated that edgesniff, a specific sniffing activity of holes edge, has complex relationships with headdip and with several components of the behavioral structure 6, 7, 8. Both frequency and duration of spontaneous elicited holepoking behavior are then measured during a short period of time. Tweet about the of mice and object detection in matlab pdf men lesson plans email the of mice and. This test is based on conflicting innate tendencies of avoidance of bright light and open spaces that ethologically mimic a situation of predator risk and of exploring novel environment. Ten days later, mice were placed in the phenotyper noldus information technology, wageningen, the netherlands for the home cage test, one animal per cage for 6 consecutive days. The faces of the mice were then photographed to understand how they express pain.
Mice were previously trained to climb a chain of 6cm long suspended from a clamp of a retort stand 100cm above ground. K 1 departments of physiology and 2pharmacology lagos state university college of medicine, p. The light intensity is not indicated but the setup used to test mice is described which varied widely. The use of the hole board in this perspective relies on the hypothesis that the behavior of animals exposed to a novel situation results from competition between an exploratory tendency and a withdrawal tendency. Due to testing order effects in a behavioral test battery, naive animals. Adult norms for the nine hole peg test of finger dexterity. This mouse strain including any and all progeny, mice is. The animal is placed on an arena with regularly arranged holes on the floor. Mice are scored daily for eae severity on the scale of 05 in 0. The apparatus mimics a small hamlet with a central agora and five houses, which are functionalized since mice can drink, eat pellets, hide within a small maze, run in an activity wheel, or interact with a stranger mouse behind a grid.
In sjl mice, eae is induced according to the protocol eae induction by active immunization in sjl mice. The test is optimally started around 4 pm if the mice are housed on an 07001900 h darklight cycle. Mice are euthanized at the appropriate stage of disease. The goal is to be able to use the test to better assess the benefits of a specific treatment on young mice. Mice are weighed 3 timesweek monday, wednesday, friday. Use of pen test balance beam to assess motor balance and. The objective of the present sop is to describe how the pen test also known as the balance beam1 can be used to evaluate the diseaserelated impairment of motor balance and coordination in mouse models of spinal muscular atrophy sma. A mouse is presented with two similar objects during the first session, and then one of the two objects. May 25, 2018 analysis of topographic memory can be performed a posteriori in a probe test by depriving mice of water or food and measuring their ability to efficiently reach the eat or drink house. Apr 08, 2015 this protocol describes the modified hole board mhb, which combines features from a traditional hole board and open field and is designed to measure multiple dimensions of unconditioned behavior in small laboratory mammals e. Of mice and men test do not write on this test form. Seven to 10 days later, the elevated plusmaze test was. Novel approach to the behavioural characterization of. Diazepam treated female mice were calm during the test while the epinephrine injected animals were nervous and freezing behavior was present.
Author links open overlay panel hiroshi takeda minoru tsuji teruhiko matsumiya. No one likes putting animals in pain, said mogil, who holds a canada research chair in the genetics of pain. An alternative masure me nt of finger dexteriry is the purdue pcgb hole board mhb is used to assess multiple dimensions of unconditioned behavior, mainly in mice and rats 1. Center for systems neurogenetics of addiction csna. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. For example, the utility of the use of rodents in testing for sepsis, burns, inflammation, stroke, als, alzheimers, diabetes, cancer, multiple sclerosis, parkinsons disease and other illnesses has been called into question by a. Of mice and men by john steinbeck summary and analysis chapter 1. The effect of diazepam on the changes in headdipping behavior in mice. Travellers attending mice activities have a purpose beyond leisure tourism, and are in fact business travellers. Here we provide instructions to measure topographic memory in mice using the hamlet test, a complex environment. The holeboard test hbt is an experimental method used in scientific research to measure.
Use of mice by forprofit entities or for commercial purposes requires a license from the scripps research institute institution prior to shipping. The classic hole board paradigm a square arena with 16 holes. Jul 14, 2005 july 14, 2005 when memory is lost due to dementia such as alzheimers disease, is it gone for good scientists probed that idea in lab tests on mice. The mice were placed at the center of the board and moved freely in the box. As to hole board assay, by means of mva, we have demonstrated that edgesniff, a specific sniffing activity of holes edge, has complex relationships with headdip and with several components of the behavioral structure 6, 7, 8.
Morse iii national institute of allergy and infectious diseases national institutes of health bethesda, maryland academic press 1978 adapted for the web by. Animal models of anxiety disorders and stress alline c. The modified hole board as a differential screen for behavior in. The partition task is another simple assay for assessing sociability in mice. Holeboard, mouse, reference memory, scopola mine, spatial learning. The holeboard hb is an explorationbased assay widely used to assess various features of anxietyrelated behaviors. The balance beam is a test of motor coordination and balance in rodents. Jul 20, 2016 collectively, these findings suggest that the battery of behavioral test buried food test, open field test and y maze test in mice could be used to study postoperative delirium in the mice. The holeboard test is mainly used for assessing exploratory behaviors in.
In both cases, the resting time was high and the program rendered similar results. Exploration bias in mice influences in the classic hole board spatial memory test. The holeboard discrimination task was conducted in test. The object recognition test is now among the most commonly used behavioral tests for mice. Please match the description of the character to each character below. Part 1 multiple choice 15 points circle the letter of. Jun 10, 2009 the staircase test of skilled reaching in mice. Changes in headdipping behavior in the holeboard test reflect the anxiogenic andor anxiolytic state in mice. Use the pdf version or alter the word version to meet your needs.
A number of widely used tests measure a single behavioral parameter which does not completely cover the. Battery of behavioral tests in mice to study postoperative. The modified hole board measuring behavior, cognition. Mice were tested in the open field, during the second part of the dark period between 1200 and 1500 h. Learn of mice and men test chapter 4 with free interactive flashcards. To match their preferred learning style and increase engagement, students are given the choice of either writing their answers on a hard copy of the the test or using their lap tops to go onto. Holeboard discrimination learning in mice request pdf. Mice have been used for decades for initial phases of testing because their genetic makeup is. Analyze the significance of the following excerpt provided on the test to the novel as a whole why is it important to the theme or main ideas of the novel. When steinbeck tells us that lennie likes to pet things, he is using the technique of. Choose from 500 different sets of of mice and men test chapter 4 flashcards on quizlet. Why is crooks actually happy when lennie and candy hang out in his room for a. The holeboard test is mainly used for assessing exploratory behaviors in rodents. In the end, why dont george and candy still buy the ranch after lennie is gone in of mice and.
This highlights the importance of ethological analysis when performing the open field test 3. Of mice and men is set in the state of lennie and george were chased from the town of weed because. Repeated exposure to an elevated maze, which consists of open areas and closed areas, may have resulted in the subjects retreating into the darker, closed arms once an initial investigation had found no potential escape routes from the apparatus. The mhb has been pharmacologically validated for both mice and rats1,6.
Assessing burrowing, nest construction, and hoarding in mice. Understand what impacts mice can bring to a destination the term mice meetings, incentives, conventions and exhibitions represents a sector of tourism which includes business events and activities. Collectively, these findings suggest that the battery of behavioral test buried food test, open field test and y maze test in mice could be used to study postoperative delirium in the mice. Neuropharmacological evaluation hole board test by gusto a research group procedure. When placed into a brightly lit open field for the first time, rats and mice tend to remain in the periphery of the apparatus or against the walls thigmotaxis. Of mice and men multiple choice test by stephanie patten tpt. This is a sensitive time to test, as they are not normally awake at this time and burrowing starts more slowly than if they were well in to the dark phase and consequently very active. Introduction drug development requires testing of novel compounds in animal models of human disease. This protocol describes the modified hole board mhb, which combines. Use of mice by forprofit entities or for commercial purposes requires a license from baylor college of medicine institution prior to shipping. The rodent bone marrow micronucleus test has been widely used as a screening procedure to detect potential chromosome damaging agents andor aneugens for over 20 years but, during this time, little work has been published to support its experimental design. Of mice and men chapter 5 quiz test your knowledge. The mice were maintained on a 12h lightdark cycle lights on at 0600 h. Assessment of topographic memory in mice in a complex.
Part 1 multiple choice 15 points circle the letter of the. This paradigm is a valuable alternative for the use of a behavioral test battery, since a broad behavioral spectrum of. Tweet about the of mice and object detection in matlab pdf men lesson. Write the letter of the correct character that matches each description. Microstructural assessment of rodent behavior in the holeboard. Holeboard discrimination learning in mice kuc 2005. A number of widely used tests measure a single behavioral parameter which does not completely cover the entire phenotype of a behavioral dimension. Novel approach to the behavioural characterization of inbred. This mouse strain including any and all progeny, mice is distributed by the jackson laboratory with the following terms and conditions. When steinbeck tells us that lennie likes to pet things, he is using the technique of a narration b b. This test also provides a simple method for assessing anxious response of a rodent to an unfamiliar environment. Pdf the exploratory behaviour of rats in the holeboard. She was going to tell george that lennie steals things c.
The exploratory behaviour of rats in the holeboard. This book does not take place in the 1930s, it takes place during the 1920s because is was during the great depression. Demonstration of the staircase test in mice as a method to assess fine motor control. Be sure to carefully read each question before answering. We gain an early insight into lennies problem when a he admits to george that he cant read traffic signs b we learn about the dead mouse in his pocket c he forgets his own name 2.
Update article animal models of anxiety disorders and stress alline c. Pdf the exploratory behaviour of rats in the holeboard apparatus. Most guidelines initially recommended 10 animals per group and immature erythrocytes ie to be. The laboratory mouse virginia godfrey, dvm, phd, dacvp university of north carolina cl davis, raleigh 2010 disclaimer this is not an aclam sanctioned presentation all information is deemed reliable and correct no warranty for accuracy no information presented is known to be specifically included in the aclam board certification exam. Meetings, incentives, conventions and exhibitions mice. While mice, rats and other rodents are by far the most widely used animals in biomedical research, recent studies have highlighted their limitations. Microstructural assessment of rodent behavior in the hole. These results suggest that changes in headdipping behavior in the holeboard test may reflect the anxiogenic andor anxiolytic state of animals. Of mice and men test page 4 of 5 part 4 short answer 10 points 2 points each answer each question to the best of your knowledge full sentences please.
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